Background: Thrombolysis is a procedure that involve the used of medication to dissolve a blood clot, the medications used in thrombolysis are called thrombolytic agents. Stone breaker (Phyllanthus niruri) is a medicinal plant with long history of folkloric therapeutic use in the treatment of pathological conditions.
Objective: this study was designed to investigate the effects of leaf Phyllanthus niruri linn) on 2-butoxyethanol-induced hemolytic thrombosis in rat model which also provides the opportunity to clinically investigate the presence of metabolites and other constituents in the body of animals.
Materials and Methods: Twenty (20) Wistar rats were divided into four groups of five wistar rats each, 100 µl of 500 mg/kg BWT of 2- butoxyethanol solution were administered intraperitoneally as a single dose followed by oral administration of P. niruri leaf extract with doses of 200 mg/kg BWT and 100ml of hydroxyl urea (HU) for 28days. In vivo antioxidant, inflammatory, apoptosis, excitatory, and thrombolysis indices were evaluated; antioxidant assays (glutathione transferase (GST), superoxide dismutase (SOD), glutathione peroxidase (GPx), reduced glutathione (GSH)), inflammatory assays (myeloperoxidase, TNF-?, IL-1β, IL-6 and xanthine oxidase), apoptotic assay (caspase-3), excitatory assay (Na+/K+ ATPase) and thrombolysis assays (Plasma and urine zinc ion concentration, Selenium determination, RNase activity in plasma; and Red cell carbonic anhydrase) activity were determined.
Result: revealed that P. Niuri leaf extract significantly ameliorated the damage induced by 2-butoxyethanol in the liver and blood of the wistar rats establishing the ameliorative potential of P. Niuri (linn.) with the effect evident of an improved thrombolysis index. The treatments reversed the oxidative effects initiated by 2-2butoxyethanol, these were revealed by increase in the activities of antioxidant assays carried out. The treatment also suppresses the effects of 2-butoxyethanol by increasing the activity of Na+/K+ ATPase, heamatological parameters checked also confirm that the leaf extract was potent enough, which showed increase in the values observed in the parameters.
Conclusion: This study has demonstrated that the extract was able to enhance the endogenous antioxidant in the administered group, inhibiting the effect of inflammation on the tissue and increasing the hematological indices declined by the 2-butoxyethanol induction, and as such would be a therapeutic agent in the management of hemolytic thrombosis diseases.
Keywords: 2-butoxyethanol, Antioxidant, Heamatology, Hemolytic thrombosis, Stone breaker
