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Assessment of relationship between hormones and insulin resistance in PCOS

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Original Article

Author Details : Manjula Halevoor Siddarajaiah, Vijayalakshmi p*

Volume : 6, Issue : 4, Year : 2019

Article Page : 584-589

https://doi.org/10.18231/j.ijcbr.2019.121

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Abstract

Introduction: Polycystic ovarian syndrome (PCOS) is the most common heterogenous disorder of
reproductive age. Increased frequency of GnRH pulses from hypothalamus elevates LH levels this in
turn causes increased androgen production. The chronic hyperandrogenic state have multiple long and
short term complications which includes DM and CVD. Insulin resistance can be characterized as impaired
action of insulin on glucose metabolism which increases risk of developing T2DM. Hyperandrogenism
with hyperinsulinemia also leads to dyslipidemia.
Aim: To estimate LH/FSH ratio, testosterone in PCOS patients and to correlate its significance with the
insulin resistance.
Materials and Methods: This case-control study was conducted on clinically, diagnosed 50 PCOS
patients, aged 15 to 35 years were included as cases. Age-matched 50 apparently heal thy women were
included as controls. Serum leutinising hormone(LH), Follicle stimulating hormone(FSH), testosterone,
serum insulin were analysed by chemiluminiscence immunoassay(CLIA) on Maglumi 1000. Mindray
BS 300, fully automated analyser was used for estimation of Total cholesterol(TC), High density
lipoprotein(HDL), Triglyceride(TG), Fasting Blood Glucose (FBG), Low density lipoprotein (LDL) was
calculated using Friedewald’s formula. Insulin resistance was assessed by HOMA IR. Descriptive statistics
analysis was done using unpaired student’s t-test. Chi-square/ Fisher Exact test has been used to find
the significance of study parameters on categorical scale. Pearson’s correlation coefficient was applied to
analyse the correlation. p value <0> Results: In our study the LH /FSH ratio, HOMA IR and Testosterone was increased in cases compared to
controls with p 0.01 and for testosterone p 0.001. In contrast HDL decreased in cases compared to controls
(p<0> and Total cholesterol were not significantly increased in cases however we can find that LDL was still
increased in cases. Average BMI was within the normal range (p=0.06). On correlation study FSH showed
a negligible negative correlation with HOMA IR and mild negative correlation with BMI. LH showed a
positive correlation with both HOMA IR(r 0.45,p<0> showed no correlation. LH/FSH ratio was positively correlated with HOMA IR(r 0.42, p<0> BMI(r 0.53, p <0> 0.21), TG(r 0.16,p 0.26) though positive showed negligible correlation with HOMA IR. BMI showed a
significant positive correlation with HOMA IR (r 0.43,p <0> Conclusion: In our study we propose that in PCOS cases IR seems to underlie many clinical features of
PCOS. It encompasses long term health problems like CVD, DM and increased exposure to estrogen can
lead to endometrial carcinoma. It is need of the time to follow up the patient to early identify and prevent
the consequences of this syndrome.

Keywords: Insulin resistance, Leutinising hormone, Follicle stimulating hormone, Dyslipidemia.

How to cite : Siddarajaiah M H, Vijayalakshmi P, Assessment of relationship between hormones and insulin resistance in PCOS. Int J Clin Biochem Res 2019;6(4):584-589

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