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Concordance between low density lipoprotein cholesterol concentration measurement by enzymatic method and calculation by Friedewald formula in cardiovascular risk classification

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Original Article

Author Details : Bénédicte Yékayo Kone Dakouri*, Amidou Toure, Marie Laure Attoungbre Hauhouot, Adele Kacou-N'douba, Ismael Namory Karamoko

Volume : 10, Issue : 3, Year : 2023

Article Page : 217-222

https://doi.org/10.18231/j.ijcbr.2023.038

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Abstract

Background: Accurate estimation of low-density lipoprotein cholesterol (LDL-C) is important for cardiovascular risk assessment and guiding cholesterol-lowering therapy. Due to the high cost of β-quantification (Gold standard) and time-consuming, direct measurement of LDL-C is an alternative method. However, unlike the calculation of LDL-C by Friedewald formula, there is an additional cost in terms of reagents for performing a direct LDL-C test. The current study aimed to compare direct LDL-C concentration determination to data calculated by Friedewald formula.
Materials and Methods: 752 lipid profiles of 710 people with LDL-C estimated by direct LDL assay, in the Biochemistry laboratory of university hospital center of Angré, were included in the study. In the same group, LDL-C was calculated using Friedewald formula. Lin’s concordance correlation coefficient (ccc) and Passing-Bablok regression analysis using, MedCal software, were performed to assess the strength of concordance between the 2 methods, and identify any possible bias.
Results: The concordance between the two methods was moderate (?c = 0.9466). Passing-Bablok regression analysis revealed a systematic bias between the two methods. The total error observed (TEobs) between the two methods was higher than allowable total error recommended by the NCEP-ATPIII when LDL-C values was less than 159 mg/dL (4.112 mmol/L).
Conclusion: The Friedewald formula resulted in lower LDL-C concentration values. Despite its cost-effectiveness in the estimation of LDL-C, an underestimation of LDL-C levels could result in inaccurate cardiovascular diseases (CVD) risk assessments and potentially significant future societal costs due to inadequate prevention and treatment of CVD.
 

Keywords: Homogeneous method, Friedewald formula, Concordance.

How to cite : Dakouri B Y K, Toure A, Hauhouot M L A, Kacou-n'douba A, Karamoko I N, Concordance between low density lipoprotein cholesterol concentration measurement by enzymatic method and calculation by Friedewald formula in cardiovascular risk classification. Int J Clin Biochem Res 2023;10(3):217-222

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