International Journal of Clinical Biochemistry and Research

Print ISSN: 2394-6369

Online ISSN: 2394-6377

CODEN : IJCBK6

International Journal of Clinical Biochemistry and Research (IJCBR) open access, peer-reviewed quarterly journal publishing since 2014 and is published under auspices of the Innovative Education and Scientific Research Foundation (IESRF), aim to uplift researchers, scholars, academicians, and professionals in all academic and scientific disciplines. IESRF is dedicated to the transfer of technology and research by publishing scientific journals, research content, providing professional’s membership, and conducting conferences, seminars, and award more...

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Original Article


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348-353


Authors Details

Shubha N. Prakash, Anitha Devanath, Ahlad


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Calibrator: How Long is it Stable?


Original Article

Author Details : Shubha N. Prakash, Anitha Devanath, Ahlad

Volume : 4, Issue : 4, Year : 2017

Article Page : 348-353


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Abstract

Calibration is a process of verifying, by comparison with a calibrator of known quantity for its accuracy and imprecision within its assay range. The calibration so performed will have validity and has to be repeated at the end of the validity. The stated stability of the calibrator material after reconstitution is 24hrs. Due to this limited stability, calibration per se, becomes a costly process since few parameters might require frequent calibration. With every calibration, new set of calibrators have to be used that would add to the cost of calibration and cost per reportable test. There are no literatures available which have studied calibrator stability beyond the manufacture’s stated stability. Since the stability of the analytes are much longer than 24 hours in serum, we wanted to see if the same is applicable in calibrators as the matrix of calibrators is comparable to serum. Calibration data from January 2015 to December 2016 was collected. All calibrators reconstituted for the analytes to be studied were included. We assayed calibrator at day 0 after reconstitution for Glucose, Total cholesterol, Total protein, Iron and Lactate dehydrogenase. The mean at 0 days was compared with the mean for 3 different time intervals beyond 24 hours after storing aliquots at -200C. The calibrator for glucose was stable for up to 8 weeks, total cholesterol for 7 weeks, total protein for 10 weeks, iron for 28 weeks and LDH for 17 weeks.

Keywords: Calibration, Calibrator, Stability


How to cite : Prakash S N, Devanath A, Ahlad, Calibrator: How Long is it Stable?. Int J Clin Biochem Res 2017;4(4):348-353

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