International Journal of Clinical Biochemistry and Research

Print ISSN: 2394-6369

Online ISSN: 2394-6377

CODEN : IJCBK6

International Journal of Clinical Biochemistry and Research (IJCBR) open access, peer-reviewed quarterly journal publishing since 2014 and is published under auspices of the Innovative Education and Scientific Research Foundation (IESRF), aim to uplift researchers, scholars, academicians, and professionals in all academic and scientific disciplines. IESRF is dedicated to the transfer of technology and research by publishing scientific journals, research content, providing professional’s membership, and conducting conferences, seminars, and award more...

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Original Article


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551-554


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Asfia Afreen*, Dinesh Javarappa, Itagappa M


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Comparative study of oxidative stress in type 2 diabetes mellitus with in vitro oxidative stress using ferrous ascorbate as pro-oxidant


Original Article

Author Details : Asfia Afreen*, Dinesh Javarappa, Itagappa M

Volume : 5, Issue : 4, Year : 2018

Article Page : 551-554

https://doi.org/10.18231/2394-6377.2018.0117



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Abstract

Diabetes mellitus is a chronic endocrine disorder associated with a multitude of microvascular and macrovascular complications. The imbalance between the productions of free radicals plays a prominent role in the development of these complications. Different markers of oxidative stress on erythrocyte membrane like Malondialdehyde (MDA), Protein carbonyl, Protein thiols and protein content of the membrane show variations which can be estimated to know the effect of oxidative stress. Similar effect is seen on erythrocyte membranes when these are subjected to proxidants like Ascorbic acid with Iron or Copper in Vitro. In our study the effect of oxidative stress on RBC membrane in diabetes was estimated and the same was validated by subjecting Erythrocyte membranes of controls to oxidative stress in vitro by using Ascorbic acid with Iron.
A case control comparative study was done with type 2 DM patient blood samples and normal controls at BMCH & RC, Chitradurga. The maximum number were of the age group of 41- 45 i.e. 32%. The mean FBS levels among cases and controls 197.50 mg/dl and 93.48 mg/dl respectively. There was similarity in the results between levels of protein content (4.56±0.19), protein carbonyl (1.20±0.08), protein thiol levels (1.42±0.10) and MDA (4.23±0.21) among diabetics in comparison to protein content (4.28±0.14), protein carbonyl (1.39±.0.097), protein thiols (1.04±0.05) and MDA (4.75±0.81) in controls treated with Iron Ascorbate a pro-oxidant mixture.

Keywords: Diabetes mellitus, Reactive oxygen species, Protein content, Protein carbonyl, Protein thiols, Iron ascorbate.


How to cite : Afreen A, Javarappa D, Itagappa M, Comparative study of oxidative stress in type 2 diabetes mellitus with in vitro oxidative stress using ferrous ascorbate as pro-oxidant. Int J Clin Biochem Res 2018;5(4):551-554

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