International Journal of Clinical Biochemistry and Research

Print ISSN: 2394-6369

Online ISSN: 2394-6377

CODEN : IJCBK6

International Journal of Clinical Biochemistry and Research (IJCBR) open access, peer-reviewed quarterly journal publishing since 2014 and is published under auspices of the Innovative Education and Scientific Research Foundation (IESRF), aim to uplift researchers, scholars, academicians, and professionals in all academic and scientific disciplines. IESRF is dedicated to the transfer of technology and research by publishing scientific journals, research content, providing professional’s membership, and conducting conferences, seminars, and award more...

  • Article highlights
  • Article tables
  • Article images

Article statistics

Viewed: 391

PDF Downloaded: 341


Get Permission Sogani and Jain: Comparison of the level of lipid peroxidative marker and antioxidants between preeclamptic and normotensive pregnant women


Introduction

Pregnancy is a stressful condition in which many physiological and metabolic functions are altered to a considerable extent.1 Preeclampsia is a pregnancy-specific hypertensive disorder sometimes progressing into a multiorgan cluster of varying clinical features affecting some 5-8% of pregnant women worldwide as a result of vasospasm and endothelial activation.2, 3 In India the incidence of preeclampsia is reported to be 8-10% of the pregnancies.4, 5 It is characterized by hypertension to the extent of 140/90 mm Hg or more, proteinuria (>300 mg/day) and pathological edema after 20th weeks of gestation.6 Despite many research, the cause of preeclampsia is not clear. Maternal symptoms are thought to be secondary to endothelial cell dysfunction. Reduced perfusion as a result of abnormal placentation leads to ischemia perfusion injury to the placenta. Placental oxidative stress, resulting from the ischemia perfusion injury, is said to be involved in the etiopathogenesis of preeclampsia.7

Preeclampsia is characterized as a state of oxidative stress resulting from increased generation of free radicals and decreased levels of antioxidants which scavenge free radicals. Oxidative stress occurs when generation of reactive oxygen species (ROS) increases and overwhelms the body’s antioxidant defenses. Reactive oxygen species is highly reactive metabolites which are derived from molecular oxygen and nitrogen. These ROS attack the phospholipids of cell membranes and react with polyunsaturated fatty acids to form lipid peroxides resulting in cellular injuries. ROS have been proposed as a promoter of lipid peroxidation and endothelial cell dysfunction.7 Thus vascular endothelial dysfunction in preeclampsia may be caused by uncontrolled lipid peroxidation. Lipid peroxidation is an oxidative process which occurs at low levels in all cells and tissues. Variety of antioxidant mechanisms serve to control this peroxidative process under normal conditions.8 Antioxidants are enzymes or compounds that scavenge and reduce the presence of free radicals. These naturally occurring antioxidants usually protects the cells and tissues from the effects of lipid peroxidation. Cumulative evidences in recent years has shown that in preeclampsia, there are an increase in lipid peroxidation and a decrease in antioxidants protection leading to oxidative stress.9 Nowadays measurement of lipid peroxidation has become an acceptable trend in medicine to consider at oxidative stress at molecular level. For this reasons, the present study was conducted to evaluate the changes in the level of lipid peroxidation markers and antioxidants in preeclamptic pregnant women and its comparison with normotensive pregnant women.

Materials and Methods

The present case control study was conducted in the Department of Biochemistry at Pacific Institute of Medical Sciences (PIMS), Umarda, Udaipur (Raj.). The 50 pregnant women were taken as subjects clinically diagnosed as preeclampsia during third trimester (28-40 weeks) with the age 18-35 years visiting obstetrics OPD and wards of M.Y. Hospital. This was done on the basis of blood pressure, (both systolic and diastolic) proteinuria and pathological edema, which are the diagnostic criteria of preeclampsia. As a control group 50 healthy normotensive pregnant women were taken who were also in the third trimester (28-40 weeks) of their pregnancy with the age 18-35 years along with the same parity and gestational age. Inclusion criteria for preeclamptic pregnant women included in the study were: should not be using any kind of oral contraceptives, anticoagulant drugs, should be non-smokers and non alcoholics and exclusion criteria was: past history of diabetes, systemic or endocrine disorder, chronic infection, chronic renal disease and hypertension women in the labor pains were excluded from the study.

Preeclampsia was diagnosed according to American college of Obstetrics and Gynecology (ACOG) criteria: a blood pressure higher than 140/90 mm Hg and proteinuria more than 300mg/24hr were observed on at least two occasions more than 6hrs apart after the 20th weeks of pregnancy.

Sample collection

Fasting ante-cubital venous blood (10ml) were obtained with aseptic measure. After clotting, the blood was centrifuged for 30 minutes and the supernatant (serum) was taken in the separate test tube. Serum for MDA, SOD, Vitamin C and Uric acid estimation was ready.

Serum MDA levels were estimated by manual method of Beuge et al.10 using TBARS method (Thiobarbituric acid reactive substances). Serum SOD levels were measured using a commercially available kit (Ransod; Randox Laboratories Ltd.) Serum Vitamin C levels were estimated calorimetrically using a DTCS reagent according to the method developed by Teitz.11 Serum Uric acid levels were estimated calorimetrically using enzymatic PAP method with uricase-peroxidase.12

Statistical analysis

All the data were expressed as mean ± SD. The statistical significance was evaluated by Student’s t-test using SPSS software, version 20. The level of significance was set at < 0.05.

Table 1

Comparison of mean and standard deviation of Anthropometric factors of control and subjects

Anthropometric factors

Healthy normotensive pregnant women (n=50)

Preeclamptic pregnant women (n=50)

Mean ± SD

Mean ±SD

Age (yrs)

23.36 ± 2.50

22.82 ± 2.82@

BMI (Kg/m2)

23.92 ± 1.61

24.12 ± 1.46@

Gestational age (wks)

38.76 ±2.92

35.74 ±2.74#

Systolic blood pressure (mm of Hg)

114.6 ± 4.98

140.2 ± 5.47#

Diastolic blood pressure (mm of Hg)

75.4 ± 5.73

91.88 ± 6.46#

Table 2

Comparison of mean and standard deviation of level of oxidative stress marker of control and subjects

Oxidative stress marker

Healthy normotensive pregnant women (n=50)

Preeclamptic pregnant women (n=50)

Mean ± SD

Mean± SD

MDA (nmol/mL)

4.09 ± 1.46

8.16 ± 1.26*

Table 3

Comparison of mean and standard deviation of levels of antioxidants of control and subjects

Antioxidants

Healthy normotensive pregnant women (n=50)

Preeclamptic pregnant women (n=50)

Mean ± SD

Mean± SD

SOD(U/mL)

4.44 ± 0.99

2.37 ± 0.64**

Vitamin C

0.88 ± 0.208

0.30 ± 0.19**

Uric acid

4.37 ± 1.30

5.49 ± 1.49**

Results

Table 1 shows the Anthropometric factors of healthy normotensive pregnant women andpreeclamptic pregnant women. When comparison of maternal age and body mass index was done between both the groups, no significant difference was observed between the groups (p>0.05, Table 1). Gestational age, systolic and diastolic blood pressures were significantly higher in preeclamptic pregnant women as compared to healthy normotensive pregnant women (p<0.001, Table 1).

Table 2 shows the comparison of levels of Oxidative stress markers between healthy normotensive pregnant women and preeclamptic pregnant women. The levels of MDA was found to be elevated in preeclamptic pregnant women when compared with healthy normotensive pregnant women and the difference was found to be highly significant between both the groups (p<0.0001, Table 2).

Table 3 shows the comparison of levels of Antioxidants between healthy normotensive pregnant women andpreeclamptic pregnant women. The levels of SOD and Vitamin C was found to be decreased and the levels of uric acid was found to be increased in preeclamptic pregnant women when compared with healthy normotensive pregnant women and the difference was found to be highly significant between both the groups (p<0.0001, Table 3).

Discussion

Preeclampsia is considered a disorder which is strongly related to oxidative stress.12 Evidences proves that in preeclampsia there is a biochemical imbalance with an increase of oxidative stress and a deficient antioxidant protection.13 It is difficult to measure free radicals as they are transient in nature and are unstable. They have a tendency to cause lipid peroxidation and have been used as an indirect measure.8 Free radicals released from the poorly perfused fetoplacental unit initiate lipid peroxidation by attacking polyunsaturated fatty acids in cell membranes, converting them to form lipid peroxidase by direct reaction of oxygen and lipids.9

Our study concluded that the levels of MDA were significantly elevated (p<0.0001) in preeclamptic pregnant women as compared to normotensive pregnant women. These findings were in consistence with the findings of other authors who also observed increased MDA levels in their studies.14, 15, 16 Lipid peroxidation is of destructive character and so its uncontrolled nature in preeclampsia is consider as an etiological factor. The products of the lipid peroxidation are of candidate factors which causes disturbance in the maternal vascular endothelium. They also inhibit prostacyclin synthesis and stimulate smooth muscle contraction resulting in widespread vasospasm, a prominent feature of preeclampsia.9 This endothelial damage ultimately increases diastolic blood pressure which further aggravates the condition of preeclamptic patients.17

Pregnancy has been characterized as an inflammatory state, and these changes are exacerbated in preeclampsia.18 A generalized activation and increased leukocytes numbers due to delayed neutrophils apoptosis contribute to leukocytosis or neutrophilia during pregnancy and preeclampsia. It contributes to endothelial dysfunction or activation in preeclampsia.19 Increased activation of WBC also exaggerates placental response which results in elevated formation of reactive oxygen species like superoxide radical which will cause increase in lipid peroxidation that damage the vascular endothelium, membrane of cells and organelles which is proved by elevated MDA level.20

Preeclampsia is a hypertensive disorder of pregnancy in which antioxidant defenses fail and tissues are injured. It has been suggested that there is a deficiency in protective antioxidant systems or increased utilization of antioxidants in preeclamptic pregnant women when compared with normotensive pregnant women. Significant decrease (p<0.001) in serum SOD level in preeclampsia has been found in the present study as compared to normotensive pregnant women. Our studies also reveal similar findings.14, 21, 22

Decreased activity of SOD is one of the hall marks of preeclampsia.23 SOD are important part of the defense system. It protects and revitalizes cells and reduces the rate of cell destruction. It is one of the most important antioxidant enzyme which is capable of neutralizing some of the most dangerous free radicals, the superoxide radicals thus preventing its excessive accumulation and may contribute to the continuation of pregnancy.8, 9, 24 It scavenges the superoxide radical by catalyzing its dismutation into H2O2 and O2.25

Several studies have demonstrated decreased serum levels of vitamin C in preeclamptic pregnant women as compared to normotensive pregnant women (p<0.0001).16, 26, 27 Our findings were in accordance with these authors. Vitamin C plays an important role in preventing lipid peroxidation. Reduced ascorbate is effective in protecting plasma lipids and susceptible molecules from peroxidation. It is observed that in preeclamptic pregnant women, antioxidants scavange the increased free radicals thus resulting in lowered levels of antioxidants.26, 28

In preeclamptic pregnancy, the oxidant/antioxidant imbalance characterizes oxidative stress which is indicated by low vitamin C levels. Vitamin C is the most important aqueous phase chain-breaking antioxidant. Antioxidant defense systems include the chain breaking antioxidants that directly scavenges radicals in membranes and lipoprotein particles and prevents lipid peroxidation. Chain breaking antioxidants present in the aqueous compartment.29, 30

Uric acid is a marker of oxidative stress, tissue injury and renal dysfunction. Abnormal tropbhoblast invasion is seen in preeclampsia due to which placenta receives less blood supply from uteroplacental artery. Placenta thus becomes hypoxic which causes placental tissue breakdown and provides additional purines source. These damaged placental tissues and placenta are the rich sources of purines for generation of uric acid by xanthine oxidase.31 This may lead to decrease in the renal tubular excretion. Thus the altered renal clearance of uric acid is due to renal dysfunction and increased xanthine oxidase activity.32

Hyperuricemia in preeclampsia is primarily due to decreased renal clearance and increased tubular reabsorption of uric acid as the glomerular filtration rate is decreased.33 Hyperuricemia also contributes to impaired endothelial integrity and contribute to the pathogenesis of preeclampsia. It has an important role in vascular damage and oxidative stress.31

Conclusions

The present study has shown a significant increase in serum MDA levels, the indicator of lipid peroxidation which suggests that lipid peroxidation plays a role on the pathogenesis of preeclampsia. Increased lipid peroxidation causes increased consumption of antioxidant free radical scavenging system. Also decreased level of SOD that causes oxidative stress, some endothelial disturbing factors like uric acid and decreased level of vitamin C is one of the pathophysiological causes. An imbalance between lipid peroxides and the antioxidant systems in preeclampsia is observed. Thus estimation of serum MDA, SOD, vitamin C and uric acid are useful in predicting the extent of endothelial damage and preventing the complications in preeclampsia. As antioxidant stress can provoke endothelial dysfunction, pregnant and preeclamptic pregnant women should be given antioxidant supplementation to prevent the overwhelming effect of oxidative stress.

Source of Funding

None.

Conflict of Interest

None.

Acknowledgment

We are highly grateful to those patients of the hospital who volunteered to donate their blood when needed for this project. Our thanks are also to the paramedical staff of the hospital for their assistance in collecting and maintaining blood samples.

References

1 

L Fialová I Malbohan M Kalousová J Soukupová L Krofta S Štípek Oxidative stress and inflammation in pregnancyScand J Clin Lab Invest2006662121710.1080/00365510500375230

2 

RA North LME McCowan GA Dekker L Poston EHY Chan AW Stewart Clinical risk prediction for pre-eclampsia in nulliparous women: development of model in international prospective cohortBMJ2011342apr07 4d187510.1136/bmj.d1875

3 

FG Cunningham NF Gant KJ Leveno LC Gilstrap JC Hauth KD Wenstrom Williams Obstetrics21st EditionMcGraw-Hill Medical Publishing Division2001568618

4 

L Poston L Chappell P Seed A Shennan Biomarkers of oxidative stress in pre-eclampsiaPregnancy Hypertens20111122710.1016/j.preghy.2010.10.009

5 

AH Khosla R Mehra D Dua P Gupta Maternal morbidity and mortality : an assessment of prevalence and aetiology factorsObs Gynae Today2006114479

6 

Diagnosis and management of preeclampsia and eclampsia. ACOG Practice Bulletin No.33. American College of Obstetricians and GynecologistsObstet Gynecol2002993315967

7 

TH Hung JN Skepper DS Charnock-Jones GJ Burton Hypoxia-reoxygenation: a potent inducer of apoptotic changes in the human placenta and possible etiological factor in preeclampsiaCirc Res20029012127481

8 

SB Patil MV Kodliwadmath SM Kodliwadmath Study of oxidative stress and enzymatic antioxidants in normal pregnancyIndian J Clin Biochem2007221135710.1007/bf02912897

9 

P Phalak J Kulkarni M Tilak AP Throat Role of lipid peroxidation and antioxidant status in pathogenesis of PreeclampsiaIndian J Basic Appl Med Res2013625369

10 

JA Buege SD Aust The thiobarbituric acid assayMethods Enzymol1978523067

11 

DB Mccormick HL Green A Carl CA Burtis R Edward ER Ashwood VitaminsTietz textbook of clinical chemistry3rd edition199910245

12 

S Gupta N Aziz L Sekhon R Agarwal G Mansour J Li Lipid Peroxidation and Antioxidant Status in PreeclampsiaObstet Gynecol Surv20096411750910.1097/ogx.0b013e3181bea0ac

13 

M Suhail MF Suhail H Khan Role of Vitamins C and E in Regulating Antioxidant and Pro-Oxidant Markers in PreeclampsiaJ Clin Biochem Nutr20084332102010.3164/jcbn.2008067

14 

PS Sheena Comparative study of oxidative stress in pregnancy induced hypertension, preeclampsia and eclampsiaInt J Biomed Adv Res2012311810410.7439/ijbar.v3i11.765

15 

J Ghate AR Choudhari B Ghugare R Singh Antioxidant role of vitamin C in normal pregnancyBiomed Res20112214951

16 

SV Kashinakunti H Sunitha DS Gurupadappa Shankarprasad G Suryaprakash JB Ingin Lipid peroxidation and antioxidant status in preeclampsiaAl Ameen J Med Sci2010313841

17 

S Aydın A Benian R Madazli S Uludaǧ H Uzun S Kaya Plasma malondialdehyde, superoxide dismutase, sE-selectin, fibronectin, endothelin-1 and nitric oxide levels in women with preeclampsiaEur J Obstet Gynecol Reprod Biol2004113121510.1016/s0301-2115(03)00368-3

18 

F Bretelle F Sabatier R Shojai New insight in physiopathology of preeclampsia and intra-uterine growth retardation; role of inflammationGyneaol Obstet Fertil2004324829

19 

BN Jahromi S Rafiee O661 Evaluation of coagulation factors in severe preeclampsiaInt J Gynecol Obstet20091073S28210.1016/s0020-7292(09)61034-1

20 

Y Yoneyama R Sawa S Suzuki D Doi K Yoneyama Y Otsubo Relationship between plasma malondialdehyde levels and adenosine deaminase activities in preeclampsiaClin Chim Acta20023221-21697310.1016/s0009-8981(02)00175-4

21 

ZH Howlader S Tamanna S Parveen HU Shekhar M Alauddin F Begum Superoxide dismutase and the changes of some micronutrients in preeclampsiaBJMS200915210713

22 

JB Sharma A Sharma A Bahadur N Vimala A Satyam S Mittal Oxidative stress markers and antioxidant levels in normal pregnancy and pre-eclampsiaInt J Gynecol Obstet200694123710.1016/j.ijgo.2006.03.025

23 

F Bernardi L Constantino R Machado F Petronilho F Dal-Pizzol Plasma nitric oxide, endothelin-1, arginase and superoxide dismutase in pre-eclamptic womenJ Obstet Gynaecol Res20083469576310.1111/j.1447-0756.2008.00860.x

24 

VM Chamy J Lepe Á Catalan D Retamal JA Escobar EM Madrid Oxidative stress is closely related to clinical severity of pre-eclampsiaBiol Res20063922293610.4067/s0716-97602006000200005

25 

X Chen RM Touyz JB Park EL Schiffrin Antioxidant Effects of Vitamins C and E Are Associated With Altered Activation of Vascular NADPH Oxidase and Superoxide Dismutase in Stroke-Prone SHRHypertension20013836061110.1161/hy09t1.094005

26 

PR Palan MS Mikhail SL Romney Placental and serum levels of carotenoids in preeclampsiaObstet Gynecol200198345962

27 

GM. Rao P Sumita M Roshni MN Ashtagimatt Plasma antioxidant vitamins and lipid peroxidation products in pregnancy induced hypertensionIndian J Clin Biochem200520119820010.1007/bf02893070

28 

S Kharb Vitamin E and C in preeclampsiaEur J Obstet Gynecol Reprod Biol200093137910.1016/s0301-2115(00)00243-8

29 

DT Lowe Nitric Oxide Dysfunction in the Pathophysiology of PreeclampsiaNitric Oxide2000444415810.1006/niox.2000.0296

30 

A Rumbold E Ota C Nagata S Shahrook CA Crowther Vitamin C supplementation in pregnancy (review).Cochrane Database Syst Rev201510.1002/14651858.CD004072.pub3

31 

A Bargale JV Ganu DJ Trivedi N Nangane R Mudaraddi A Sagare Serum hs-CRP and Uric acid as indicator of severity in preeclampsiaInt J Pharma and Bio Sci2011233405

32 

S Sahu M Daniel R Abraham R Vedavalli V Senthilvel Study of uric acid and nitric oxide concentrations in preeclampsia and normal pregnancyInt J Biol Med Res2011213903

33 

B Dane Z Kayaoglu C Dane G Batmaz M Kiray Y Doventas The relationship between elevated maternal uric acid level and bilateral early diastolic notching at uterine arteries at second trimester and pregnancy complicationsPerinat J20111926470



jats-html.xsl


This is an Open Access (OA) journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.

Article type

Original Article


Article page

87-91


Authors Details

Sonal Sogani*, Suman Jain


Article History

Received : 13-05-2021

Accepted : 01-06-2021


Article Metrics


View Article As

 


Downlaod Files