This article is a general review of personal experience of my journey from the setup of clinical biochemistry lab and final accreditation by NABL national accreditation board for testing laboratories.) This general review can useful for post graduates in clinical biochemistry and young clinical biochemists for guidelines to start up a newclinical biochemistry lab. Planning and setup diagnostic clinical biochemistry lab needs requirements like Resource requirement, Equipment procurement and Scope of tests. Quality management system contains Internal quality control, External quality assurance scheme (EQAS). Continous improvement plan need for Technology update and Scope expansion. Accreditation process starts from accreditation body and standards and implementation of standards. Planning and startup of clinical biochemistry lab needs proper resource and equipment requirement along with support fromtechnical staff for preparing documents and implementation. Finally, for accreditation process requires timelines and encouragement from management authorities.